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pbluescript (pbs) ii sk  (Agilent technologies)


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    Agilent technologies pbluescript (pbs) ii sk
    Pbluescript (Pbs) Ii Sk, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+sk++(pbs)/us11732247-282-25-31
    Average 90 stars, based on 1 article reviews
    pbluescript (pbs) ii sk - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Method of producing lipid
    Article Snippet: Specific examples of the vector that can be used preferably in the present invention include, in the case of using a microorganism as the host, pBluescript (pBS) II SK(−) (manufactured by Stratagene), a pSTV-based vector (manufactured by Takara Bio), a pUC-based vector (manufactured by Takara Shuzo), a pET-based vector (manufactured by Takara Bio), a pGEX-based vector (manufactured by GE Healthcare), a pCold-based vector (manufactured by Takara Bio), pHY300PLK (manufactured by Takara Bio), pUB110 (1986, Plasmid 15(2), p. 93-103), pBR322 (manufactured by Takara Bio), pRS403 (manufactured by Stratagene), pMW218/219 (manufactured by Nippon Gene), a pRI-based vector (manufactured by Takara Bio), a pBI-based vector (manufactured by Clontech), and an IN3-based vector (manufactured by Inplanta Innovations Inc.).

    Article Title: Highly Efficient Transgenesis in Ferrets Using CRISPR/Cas9-Mediated Homology-Independent Insertion at the ROSA26 Locus.
    Article Snippet: To generate the transgene donor plasmid, the transgene cassette was cloned into pBlueScript SK II (+) (pBS-SKII) backbone plasmid (Agilent Technologies, La Jolla, CA) by multiple subcloning steps.

    Article Title: Method of producing lipid
    Article Snippet: Specific examples of the vector that can be used preferably in the present invention include, in the case of using a microorganism as the host, pBluescript (pBS) II SK(−) (manufactured by Stratagene), a pSTV-based vector (manufactured by Takara Bio), a pUC-based vector (manufactured by Takara Shuzo), a pET-based vector (manufactured by Takara Bio), a pGEX-based vector (manufactured by GE Healthcare), a pCold-based vector (manufactured by Takara Bio), pHY300PLK (manufactured by Takara Bio), pUB110 (McKenzie, T. et al., 1986, Plasmid 15(2), p. 93-103), pBR322 (manufactured by Takara Bio), pRS403 (manufactured by Stratagene), and pMW218/219 (manufactured by Nippon Gene).

    Article Title: Method of producing lipid
    Article Snippet: Specific examples of the vector for gene expression that can be preferably used in the present invention include, in the case of using a microorganism as the host, pBluescript (pBS) II SK(−) (manufactured by Stratagene), a pSTV-based vector (manufactured by Takara Bio), pUC-based vector (manufactured by Takara Shuzo), a pET-based vector (manufactured by Takara Bio), a pGEX-based vector (manufactured by GE Healthcare), a pCold-based vector (manufactured by Takara Bio), pHY300PLK (manufactured by Takara Bio), pUB110 (Mckenzie, T. et al., (1986), Plasmid 15(2); p. 93-103), pBR322 (manufactured by Takara Bio), pRS403 (manufactured by Stratagene), and pMW218/219 (manufactured by Nippon Gene).

    Article Title: Mycosin-1, a subtilisin-like serine protease of Mycobacterium tuberculosis , is cell wall-associated and expressed during infection of macrophages
    Article Snippet: E. coli XL-1 Blue (Stratagene) was used as host strain for transformations of the vectors pGEX-2T (Pharmacia), p19Kpro (a gift from Koen De Smet, Imperial College Medical School at St Mary's, London, UK), and pBluescript SK (pBS) (Stratagene). pGEX-2T is an E. coli expression vector for the expression of a heterologous protein with an N-terminal GST fusion partner, and p19Kpro is a mycobacterial expression vector derived from p16R1 with a constitutive 19 kDa antigen promoter [ ].

    Article Title: Aquaporin regulates cell rounding through vacuole formation during endothelial-to-hematopoietic transition
    Article Snippet: The PCR products were cloned into pBluescript II-SK (pBS, Stratagene).

    Article Title: Method of producing lipid
    Article Snippet: Specific examples of the vector that can be used preferably in the present invention include, in the case of using a microorganism as the host, pBluescript (pBS) II SK(−) (manufactured by Stratagene), a pSTV-based vector (manufactured by Takara Bio), a pUC-based vector (manufactured by Takara Shuzo), a pET-based vector (manufactured by Takara Bio), a pGEX-based vector (manufactured by GE Healthcare), a pCold-based vector (manufactured by Takara Bio), pHY300PLK (manufactured by Takara Bio), pUB110 (1986, Plasmid 15(2), p. 93-103), pBR322 (manufactured by Takara Bio), pRS403 (manufactured by Stratagene), and pMW218/219 (manufactured by Nippon Gene).

    Cloning:

    Article Title: A Tale of Two Transitions: The Unfolding Mechanism of the prfA RNA Thermosensor.
    Article Snippet: RNA thermosensors (RNATs), found in the 5′ untranslated region (UTR) of some bacterial messenger RNAs (mRNAs), control the translation of the downstream gene in a temperature-dependent manner.. In Listeria monocytogenes, the expression of a key transcription factor, PrfA, is mediated by an RNAT in its 5′ UTR.. PrfA functions as a master regulator of virulence in L. monocytogenes, controlling the expression of many virulence factors.

    Plasmid Preparation:

    Article Title: A Tale of Two Transitions: The Unfolding Mechanism of the prfA RNA Thermosensor.
    Article Snippet: RNA thermosensors (RNATs), found in the 5′ untranslated region (UTR) of some bacterial messenger RNAs (mRNAs), control the translation of the downstream gene in a temperature-dependent manner.. In Listeria monocytogenes, the expression of a key transcription factor, PrfA, is mediated by an RNAT in its 5′ UTR.. PrfA functions as a master regulator of virulence in L. monocytogenes, controlling the expression of many virulence factors.



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    (A) A pBluescript II SK+ vector was chosen as a PCR template for amplifying the indicated lengths of fragments. Both PCR products, depicted in blue and red, were combined and assembled into the intact pBluescript II SK+ vector using SLIC techniques. The black lines represent the 15 bp complementary sequences of each product. (B) A schematic diagram of phosphodiester and phosphorothioate (PT) bonds used in the preparation of primer synthesis. (C) Primers for amplifying the two different regions of pBluescript II SK+ in (A) were modified with a 5’ end phosphate (P-15 mer-15 mer), a 5’ end phosphate together with five PTs (P-15 mer-5PT-10 mer), five PTs only (15 mer-5PT-10 mer), or were not modified (15 mer-15 mer). The orange braces represent the complementary sequences. (D) Transformation efficiencies were calculated by counting the number of colonies in triplicate experiments. 1×10 8 competent cells were used for transforming the mixture of 50 ng of each purified PCR product after undergoing SLIC experiments. Single asterisk; P-values lower than 0.05, double asterisk; lower than 0.01, triple asterisks; P-value lower than 0.001. All experiments were repeated three times. (E) Representative plates of (D) are shown. CFU; colony forming unit, Ctl; control, Lambda Exo; lambda exonuclease, T5 exo; T5 exonuclease.

    Journal: PLOS ONE

    Article Title: DAPE cloning with modified primers for producing designated lengths of 3’ single-stranded ends in PCR products

    doi: 10.1371/journal.pone.0318015

    Figure Lengend Snippet: (A) A pBluescript II SK+ vector was chosen as a PCR template for amplifying the indicated lengths of fragments. Both PCR products, depicted in blue and red, were combined and assembled into the intact pBluescript II SK+ vector using SLIC techniques. The black lines represent the 15 bp complementary sequences of each product. (B) A schematic diagram of phosphodiester and phosphorothioate (PT) bonds used in the preparation of primer synthesis. (C) Primers for amplifying the two different regions of pBluescript II SK+ in (A) were modified with a 5’ end phosphate (P-15 mer-15 mer), a 5’ end phosphate together with five PTs (P-15 mer-5PT-10 mer), five PTs only (15 mer-5PT-10 mer), or were not modified (15 mer-15 mer). The orange braces represent the complementary sequences. (D) Transformation efficiencies were calculated by counting the number of colonies in triplicate experiments. 1×10 8 competent cells were used for transforming the mixture of 50 ng of each purified PCR product after undergoing SLIC experiments. Single asterisk; P-values lower than 0.05, double asterisk; lower than 0.01, triple asterisks; P-value lower than 0.001. All experiments were repeated three times. (E) Representative plates of (D) are shown. CFU; colony forming unit, Ctl; control, Lambda Exo; lambda exonuclease, T5 exo; T5 exonuclease.

    Article Snippet: 10 ng of pBluescript II SK+ was used as the PCR template. pX330-puro was a gift from Sandra Martha Gomes Dias (Addgene plasmid # 110403; http://n2t.net/addgene:110403 ; RRID:Addgene 110403).

    Techniques: Plasmid Preparation, Modification, Transformation Assay, Purification, Control